A series of six to nine consecutive histidine residues can bind to immobilized m

Question

A series of six to nine consecutive histidine residues can bind to immobilized metals such as nickel (Ni). The purification of a protein containing six histidines at its C-terminus using a nickel-based resin would be achieved by _____ chromatography. size exclusion. cation exchange. hydrophobicity. affinity. anion exchange. A series of six to nine consecutive histidine residues can bind to immobilized metals such as nickel (Ni). The purification of a protein containing six histidines at its C-terminus using a nickel-based resin would be achieved by _____ chromatography. size exclusion. cation exchange. hydrophobicity. affinity. anion exchange.

Explanation / Answer

The answer is Affinity chromatography.

Immobilized metal affinity chromatography (IMAC) is most frequently used chromatography method to seperate the protein of interest. It results 100 fold enrichment in single purification step. So, to seperate the POI from other genes, the products recombinant proteins will be tagged with 9X or 6X his tags either at C or at N terminus.

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