A series of cell culture samples, each expected to have different Ca^+2 expressi

Question

A series of cell culture samples, each expected to have different Ca^+2 expression levels due to their different states of hypoxia, were analyzed using two different techniques; fluorescence ativated cell sorting (FACS) and fluorescence resonance energy transfer (FRET). The results of the Ca^+2 expression levels from the FACS analyses provided values of

Sample 1= 3.2 mM

Sample 2= 12.8 mM

Sample 3= 7.3 mM

Sample 4= 1.0 mM

Sample 5= 5.4 mM

Sample 6= 6.1 mM

Sample 7= 2.2 mM

The results from the FRET analyses for the exact same 7 samples revealed values of;

Sample 1= 2.8 mM

Sample 2= 12.6 mM

Sample 3= 7.7 mM

Sample 4= 1.2 mM

Sample 5= 5.5 mM

Sample 6= 5.6 mM

Sample 7= 2.7 mM

Based on these results , is the accuracy of the FRET analysis method the same as that of the FACS analysis method at the 95% confidence level?

Explanation / Answer

Accuracy for samples 2 , 3 , 4 and 5 for FRET Analysis is same as that of FACS analysis method at 95% confidence level. But for sample 1 accuracy is less and confidence levell has to go down to 86% for Sample 6 accuracy is around 80% and for sample 7 it is around 78% . So for these three samples there is a difference in the accuracy of FRET and FACS analysis method

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