The electrophoresis gel shown below in part (a) is from a DNase I footprint anal

Question

The electrophoresis gel shown below in part (a) is from a DNase I footprint analysis of an operon transcription control region. DNA sequence analysis of a 35-bp region is shown in part (b). The control region, labeled with 32P at one end, is shown in a map in part (c). Separate samples of control-region DNA are exposed to DNase I, and the resulting DNase I-digested DNA is run in separate lanes of the electrophoresis gel. Unprotected DNA is in lane 1, DNA protected by repressor protein is in lane 2, and RNA polymerase-protected DNA is in lane 3. The numbers along the electrophoresis gel correspond to the 35-bp sequence labeled on the map in part (c). Use the information provided to solve the following problems.

Determine the DNA sequence of the 35-bp region examined.

Express your answer as a sequence of nucleotides. Example: TGACTAAGC.

Part B

Choose the region of the sequence protected by repressor protein.

E. 1-20

Part C

Choose the region of the sequence protected by RNA polymerase.

Choose the region of the sequence protected by RNA polymerase.

A. 31-35 B. 1-35 C. 20-31 D. 1-20 and 31-35

E. 1-20

Part C

Choose the region of the sequence protected by RNA polymerase.

Choose the region of the sequence protected by RNA polymerase.

A. 1-35 B. 5-23 C. 23-35 D. 1-5 E. 1-5 and 23-35 (b) DNA sequencing (a) Phasel treatment ???? 30 20 20 10? 5- 35

Explanation / Answer

the sequence of DNA was as follows

TGA TCA AAT ACA GTT ACA CTT ACA ATC AAG ATT C

for your conviniace i had gone for the spacing between the code but there will be no space in between them

2)protein that inhibits the expression of one or more genes by binding to the operator or associated silencers which prevent the transcrption of the genes in mRNA so the gel will show the sequence which was not under goes transcription so the answer will be D

3) E thes was protected from the RNA polymerase

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