21. The lysis solution contained which of the following? a) primers b) nucleotid

Question

21. The lysis solution contained which of the following? a) primers b) nucleotides )DNA polymerase d) EDTA 22. The PCR step in each cycle that occurs at -61 degrees Celsius is typically b) elongation c) amplification d) annealing e) none a)denaturing 23. After the first centrifugation, the DNA was found primarily in... c) both of these d) neither of these a)the supernatant b)the pellet True a, False b 24. Transposons 25. The mode of insertion of a retrotransposon ultima are flanked by both inverted repeat sequences and also direct repeated sequences. tely results in an overall increase in the number of copies of the retrotransposon in the genome. The technique used this given week in lab is the same technique used in paternity two. 26. tests and crime scene investigations, except that more loci are used in these latter Lab ulation Multiple Choice 27. During gel electrophoresis, DNA runs toward theend. a) positive b) negative c) neutral d) all of these e) none of these 28. When visualizing DNA, ethidium bromide (or Gelstar) a) attaches to the phosphate backbone. b) surrounds the DNA c) inserts in between the bases of the DNA double helix. d) replaces G bases of the DNA e) replaces C bases of the DNA when using 50mL of TAE buffer to create a 1.5% agarose gel, how many grams of agarose should be measured? 29, ?) 3 grams b)0.15 grams c)1.5 grams d) 7.5 grams e) 0.75 grams

Explanation / Answer

21. d.EDTA

Other components are not part of lysis buffer

22.d.annealing

23.b.the pellet

In the first step after centrifugation nucleus is pelleted down

24.True

DNA transposons have inverted terminal inverted repeats, and are also mostly flanked by short direct repeats of host DNA,

25.true

The retrotransposons increases the copy numbers of elements and thereby can increase genome size

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