A partial diploid was generated by transforming E. coli with a plasmid containin

Question

A partial diploid was generated by transforming E. coli with a plasmid containing lacI, the promoter and operator for the lac operon, and the lac operon. The genotypes of the partial diploid mutants with respect to the lac operon are shown below. +, indicates wild-type, -, indicates mutant, c represents constitutive, s represents no binding to inducer, i represents lacI, p represents the lac operon promoter, o represents the lac operon operator, z represents lacZ, y represents lacY, and a represents lacA.

Which strain(s) will show the listed phenotypes? If no strain will show the phenotype, please indicate as “none”.

I. constitutive production of ?-galatosidase, but normal production of permease and transacetylase. ___________

II. super-repression of the lac operon ________

III. constitutive expression of the three genes within the lac operon ___________

Explanation / Answer

1. Starin 3 shows constitutive production of beta galactosidase and normal for lac Y and lac A

2. None , super repression will not occur

3. Strain 1 , Constitutive expression of lac A, Y and A

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