The role of E. coli plasmids in recombinant DNA work is to: Question 9 options:

Question

The role of E. coli plasmids in recombinant DNA work is to:

Question 9 options:

a) 

Serve as a host for a "foreign" gene.

b) 

Provide restriction enzymes needed for repairing defective DNA.

c) 

Splice DNA strands together to form circular DNA molecules.

d) 

Cleave double-stranded DNA molecules.

Question 10 (2 points)

 
The codon ACG encodes the amino acid threonine.  What is the sequence of the anticodon that is complementary to this codon?

Question 10 options:

a) 

5'-TGC-3'

b) 

3'-UGC-5'

c) 

3'-TGC-3'

d) 

5'-UGC-3'

Question 11 (2 points)

Consider the following DNA strand: ATCCGGTAA.  How many hydrogen bonds hold this strand and its complementary strand together?

Question 11 options:

a) 

9 hydrogen bonds

b) 

18 hydrogen bonds

c) 

22 hydrogen bonds

d) 

23 hydrogen bonds

Question 12 (2 points)

Consider a nucleotide that contains a triphosphate, ribose, and the base cytosine (C).  This nucleotide would be used to synthesize:

Question 12 options:

a) 

Neither DNA or RNA

b) 

RNA only

c) 

DNA only

d) 

Both DNA and RNA

Explanation / Answer

9. The role of E. coli plasmids in recombinant DNA work is to:

ANS: c) Splice DNA strands together to form circular DNA molecules.

10. The codon ACG encodes the amino acid threonine. What is the sequence of the anticodon that is complementary to this codon?

ANS: b) 3'-UGC-5'

Explanation: A codes U, C codes G and G codes C.

Anticodon always resent in 3’--> 5’ direction.

11. Consider the following DNA strand: ATCCGGTAA. How many hydrogen bonds hold this strand and its complementary strand together?

ANS: c) 22 hydrogen bonds

Explanation: In between A and T 2 hydrogen bonds and in between G and C 3 hydrogen bonds are present.

Total number of A and T nucleotides are 5, so 5 x 2 =10

Total number of G and C nucleotides are 5, so 4 x 3 =12

12. Consider a nucleotide that contains a triphosphate, ribose, and the base cytosine (C). This nucleotide would be used to synthesize:

ANS: b) RNA only

Explanation: This nucleotide would be used to synthesize only RNA, because the given sugar ribose was ony present in RNA. The DNA can contains deoxy ribose sugar.

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