PART B:(9 Marks) A small 7 am manipulations: ino acid peptide was subjected to t

Question

PART B:(9 Marks) A small 7 am manipulations: ino acid peptide was subjected to the following sequencing peptide was subjected to amino acid compositional analysis. The results showed that it contained equal amounts (moles) of the amino acids Tyr, Pro, ALA . Asp, Gly, Lys, Ser. i) Carboxypeptidase digestion (which has C-terminal amino acid specificity) of ii) Prolyl endopeptidase (which cleaves after Pro) treatment of the peptide also iv) Digestion of the peptide with V8 protease (which cleaves after Glu or Asp) v) Amino acid compositional analysis of peptide A detected Asp, Ser, Lys and the peptide yielded free Ala. released free Ala. yielded 2 fragments designated peptide A and peptide B. Gly. Amino acid compositional analysis of peptide B detected Pro, Tyr and Ala. vi) Analysis of peptide A with PITC (Edman Reagent) yielded the PTH hydrantoin derivative of Gly vii) Digestion of the peptide A with trypsin (which cleaves after Lys or Arg) split peptide A in half giving two equal sized peptides. Predict the amino acid sequence of the peptide? Rough work paper is provided at the end of this exam script. Sequence:

Explanation / Answer

Amino acid composition = Tyr, Pro, Ala, Asp, Gly, Lys, and Ser

N-terminal amino acid = Gly

C-terminal amino acid = Ala

Prolyl endopeptidase: Pro-Ala

Peptide B = Tyr-Pro-Ala

Peptide A = Gly-Lys-Ser-Asp

Protein sequence = NH2-Gly-Lys-Ser-Asp-Tyr-Pro-Ala

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.