what is gery Value? 140 _Water RNV[Rhodamine B] 70 550 600 650 700 PLGA Shell Wa

Question

what is gery Value?

140 _Water RNV[Rhodamine B] 70 550 600 650 700 PLGA Shell Wavelength (nm) 140 40 -Water _RNV[Calcein] C 70 20 630 720 Wavelength (nm) NA R Figure 1. Characterization of the RNV encapsulating calcein, rhod amine B and siRNA. A) TEM image of a hollow core-shell RNV. Scale bar, 50 nm. B) Emission spectrum of RNV loaded with calcein excited at 455 nm. C) Emission spectrum of RNV loaded with rhodamine B excited at 556 nm. D) Gel retardation graph of free siRNA, lysed RNVIS? RNAI, and RNVIsi RNAL Lysed RNV refers to the RNV destroyed by three cycles of freeze-thaw and sonication to release the siRNA inside the water core

Explanation / Answer

If we look at the description of Figure D, it is given that the lysed RNV[si RNA] is the RNV destroyed by three freeze-thaw & sonication cycles to release the siRNA inside the water core (Assuming the water core does not have any free siRNA).

So according to the Gel retardation graph (Figure 1 D), the Gray value inside the water core ranges from 95-110 (siRNA in RNV).

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