1) Which of the following would be the worst primer for PCR? a. A primer with mi
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Question
1) Which of the following would be the worst primer for PCR?
a. A primer with mismatches at the 5' end.
b. A primer that is 21 bases long.
c. A primer with mismatches in the middle.
d. A primer with an AGG at the 3' end.
e. A primer with mismatches at the 3' end.
2) What is the purpose of PCR?:
a. reverse transcribing RNA into DNA
b. amplifying proteins
c. digesting proteins
d. copying plasmids
e. amplifying DNA
3)
Which of the below statements IS true about the process of PCR?
Select one:
a. During PCR, the primers will be extended from the 3' end.
b. The reverse primer is identical to the reference strand.
c. The forward primer binds to the reference strand.
d. During PCR, the primers will be extended from the 5' end.
e. The reverse primer is extended in the 5' to 3'direction following the reference strand.
Explanation / Answer
1. The primer with mismatch at the 3' end would be the worst primer for PCR.
2. The purpose of PCR is amplifying DNA.
3. The reverse primer is extended in the 5' to 3' direction following the reference strand. Is true about PCR process.
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