27-30 Enrichment techniques are a common method in culture-dependent study of mi

Question

27-30 Enrichment techniques are a common method in culture-dependent study of microorganisms Consider the following enrichment experiment used to isolate nitrogen-fixing bacteria. Indicate whether you would observe growth (A) or no growth (B) on each of the agar media plates 2 points each. The absence of NH4. forces cells to fix Ny Such cells can also use NH,' if available 27. Mineral salts medium containing mannitol but lacking NH4". NO r organie nitrogen 28. Incubate Soilaerobically 29. NH4 NH4" plate 30 NH4" plate Adding NH4 to selects against N-fixing bacteria.

Explanation / Answer

Answer

27, 28 - GRow
Place soil into a flask containing nitrogen free broth, with no nitrogen source in the broth, only bacteria in the soil that can fix N2 will be able to grow.

29, 30 - No growth

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