1. How can one utilize a systems biology approach in combination with RNA interf

Question

1. How can one utilize a systems biology approach in combination with RNA interference approach to understand gene function and/or define components of pathways?

2. In general, describe the principles and mechanisms for CRISPR-Cas9 system design and function

3. How would you use the system to delete a specific region, change nucleotide sequence in a specific region, or add specific sequences to a region?

4. How can one check to make sure that the intended modification actually occurred after performing genome engineering using this system.

Please answer all questions. Thank you so much

Explanation / Answer

1. System biology along with RNAi used to find the function of many genes. In drosophila, study has to find the proteins involved in the signal transduction of drosophila. The proteins are identified using topology of signal transductuon pathway. Topological parameters include betweeness centrality .Degree of the network. After, then RNAi is used to find the function of genes that are derived using signal transduction pathway .

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