Do additions of the chemicals listed to the right of the table induce, inhibit,

Question

Do additions of the chemicals listed to the right of the table induce, inhibit, or intensify the actions of Bax? blem Bcl-2 sruption cytochrome c mutation), fibrob stern blotting EXPERIMENT The mechanism of action of a Bax-binding, apoptosis-inducing compound (designated 106 in the figure) was studied in this experiment. A mitochondrial fraction was isolated from Bak /Bax mouse embryonic fibroblasts, resuspended in an appropriate buffer, and aliquots of it were treated with the agents indicated in the figure. (Note: Bcl-XL is an antiapoptotic; Bak and Bax are multidomain proapoptotic; and tBid is a BH3-only member of the Bcl-2 protein family.) The mixtures were centrifuged to pellet mitochondria, and the pellets (P) and supernatants (S) were subjected to Westem blotting using anti-cytochrome c and anti-Tom 40 antibodies. (Note: Tom 40 is an outer mitochondrial membrane protein.) FIGURE + + Bcl-XL (0.5 ?M) --106 (1 uM) - tBid (2.5 nM) PSPS PS PS PSPS PS cytochrome c Tom 40 1 2 34 5 6 7 8 9 10 11 12 1314 do adoitions of the onemi cals rsteot n , inhibit or nte l .py ? he actio~ of Bax.,

Explanation / Answer

Answer

Compound 106 stimulates the translocation of Bax protein into the mitochondria.
Treatment of isolated mitochondria with the test compound in this study led to tBid-independent insertion of the Bax protein into the outer mitochondrial membrane.

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