Once the DNA has been collected from any numl has been collected from any number

Question

Once the DNA has been collected from any numl has been collected from any number of sources, including blood, semen, skin, will be digested" by restriction enzymes. These are enzymes that were originany isolated from bacteria that recognize a specific sequence of a that recognize a specific sequence of bases and cut the DNA at each one of those sequences. For example, the restriction enzyme Haelll was isola bacterium Haemophilus aegypticus and recognizes the following sequence: ? Go s, the restriction enzyme HaellI was isolated from the sample of DNA was mixed with HaellI, it would result in what is referred to as digested recognizes the following sequence: 5'GGCC3'. If a DNA” and your resulting DNA will be in smaller fragments. The size of the fragments will be determined by where the recognition sequence was and how many times it was present. See Figure 8.1. Restriction enzymes can create two kinds of cuts in DNA. One type of cut creates what is known as "blunt ends"; this is where the restriction enzyme cuts straight across the double helix. The second type of restriction enzyme will cut across the helix in an offset fashion creating what are referred to as "sticky ends." These so-called sticky ends are just pieces of DNA with a short single-stranded end where the cut was made. EcoRI is a well-known restriction enzyme that produces these kinds of cuts and it recognizes the sequence 5'GAATTC3'. See Figure 8.2. 2. What would be the point of digesting" two different sources of DNA with a restriction enzyme that produces "sticky ends"? Assume that both sources of DNA are cut with the same restriction enzyme.

Explanation / Answer

The sticky ends generated by digesting two different sources of DNA with same restriction enzyme would form base pairs, being complimentary in nature. The fragments can then be sealed with the enzyme DNA ligase to form a recombinant DNA molecule. The recombinant DNA can be transferred to a suitable host for cloning and obtaining desired gene product.

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