1) You are interested in gene X and analyzed gene X by PCR (with enzyme digestio
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1) You are interested in gene X and analyzed gene X by PCR (with enzyme digestion), a Northern blot (ie, looking at all transcripts corresponding to gene X), a Western blot (i.e. looking at all proteins corresponding to geneX M (bp) WT 1 M (bp) WT 1 2 M(kD) WT 123 PCR gel RNA blot protein blot A. What, if any, gene X mutations are there in unknowns 1, 2, and 3? What type of possible mutation is present (i.e., frameshift, nonsense, deletion, etc.) B. Which would you expect phenotypically for unknowns 1, 2, and 3 (1 e, would you expect the unknowns to be wild type, show a phenotype, etc.)? 2) You are following four traits (a, b, c, and d) The following crosses were performed Cross 1: a b female x c d male Fl progeny: all wild typeExplanation / Answer
In this picture three images are given first is gene digestion second is RNA and third is protein. in this take each step one by one
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I these mutation phenotypically only first gene is unable to detect because that is occur due to change of single base pair change. This mutation only detects by sequencing or change in restriction digestion pattern.
Rest gene mutation can be detected due to intermediate termination and truncation they will not express the wild type phenotype.
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