How will you create loss-of-function alleles of the mouse genes? Drag the terms

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How will you create loss-of-function alleles of the mouse genes? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all Reset Help flanking an essential Hoxi Loss-of-function alleles of Hoxa7 and Hoxb7 could be created by performing gene knockouts for each exon gene. The gene knockout would be performed by creating a transgene that contains DNA corresponding to the internal region of an essential Hox7 exon with a selectable marker in the place of an exon. A selectable marker would be included outside the DNA Injection of this construct into mouse cells and selection for the selectable marker and against the positive selectable marker would yield clones containing the knockout of negative These cells would then be used to generate mice that carry the knockout construct in egg their cells sperm ES germ line at least one Hox7 gene both Hox7 genes

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Loss-of-function alleles of Hoxa7 and Hoxb7 could be created by performing gene knockouts for each gene. The gene knockout would be performed by creating a transgene that contains DNA flanking an essential Hox7 exon with a Positive selectable marker in the place of an exon. A negative selectable marker would be included outside the DNA flanking an essential Hox7 exon. Injection of this construct into mouse ES cells and selection for the positive selectable marker and against the negative selectable marker would yield clones containing the knockout of at least one Hox7 gene. These ES cells would then be used to generate mice that carry the knockout construct in their germ line cells.

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