5. Genes have NOT been successfully introduced (“spliced”) into: a. Prokaryotes

Question

5. Genes have NOT been successfully introduced (“spliced”) into:

a. Prokaryotes

b. Plants

c. Animals

d. None of the above

6. Restriction enzymes:

a. Were originally isolated from bacteria

b. Only cut DNA at random sequences

c. Can cut DNA at specific sequences

d. Always leave blunt ends

e. A and C

7. A proper Watson-Crick DNA base pair is:

a. A-G

b. C-T

c. A-T

d. None of the above

8. “Sticky ends” allow DNA fragments to be spliced into vectors by what type of bonding?

a. Ionic

b. Covalent

c. Hydrogen

d. None of the above

9. The enzyme ligase:

a. Cuts DNA and creates blunt ends

b. Cuts DNA and creates sticky ends

c. Completes the splicing of DNA fragments and plasmid vectors

d. None of the above

10. You cut a vector with the following DNA base overhang (“sticky end”): ACCTGGACTTA CCTGAAT You will need to cut out the DNA fragment containing your gene of interest so that which sticky end is created?

a. XXXXXXXX XXXXXXXXTGGA

b. XXXXXXXX XXXXXXXXACCT

c. XXXXXXXX XXXXXXXXTCCA

d. XXXXXXXX XXXXXXXXATTC

Explanation / Answer

Answer:

5.

Genes have successfully sliced into all of the organisms.

Hence,option (d) is correct.

6.

Restriction enzymes were originally isolated from bacteria and It can cut DNA at specific sequences.

Hence,option (e) is correct.

7.

A Proper Watson-Crick DNA base pair is A-T,G-C.

Hence,option (C) is correct.

8.

Sticky ends” allow DNA fragments to be spliced into vectors by hydrogen bonding.

Hence,option (C) is correct.

9.

The enzyme ligase completes the splicing of DNA fragments and plasmid vectors.

Hence, option (C) is correct.

10.

Option (d) is correct answer.

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