The student transforms and grows several colonies of their recombinant plasmids

Question

The student transforms and grows several colonies of their recombinant plasmids after this component sticks them together. The student wants to determine which colony has the correct Evan1 gene inserted into the plasmid. They remember their PI mentioning that the 1kb Evan1 gene has a BamHI site right in the middle of it. The student decides to use this restriction enzyme to test for the presence of the correct colony. How many bands would the student expect to find? What are the sizes of each of these bands?

Explanation / Answer

Given information,

Size of Evan1 = 1 kb

It contains a BamHI site in the middle.

So, BamHI digestion of Evan1 gene produces a single band with the size 500 bp.

This gene is cloned into a vector. If the vector does not contain any other BamHI sites, Restriction digestion with BamHI linearizes the vector. The linearized vector size = Vector size + 1 kb

Depending upon the number and location of BamHI sites, the digestion pattern changes.

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