Wild-type E. coli grow best at 37 but can grow efficiently up to 42 . An E. coli

Question

Wild-type E. coli grow best at 37 but can grow efficiently up to 42 . An E. coli strain has a mutation of the sigma subunit that results in an RNA polymerase holoenzyme that is stable and transcribes at wild-type levels at 37 . The mutant holoenzyme is progressively destabilized as the temperature is raised, and it completely denatures and ceases to carry out transcription at 42 . Relative to wild-type growth, characterize the ability of the mutant strain to carry out transcription at: 37 degrees A. organism does not transcribe gene, B. organism transcribes slowly, C. organism transcribes a wild type, D. organism transcribes faster than a wild type 2. at 40 degrees 3. At 47 degrees 4. What term best characterizes the type of mutation exhibited by the mutant bacterial strain?

Explanation / Answer

4. Temperature sensitive

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