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I a classical gonetic analysis, sciontists create mutations wthin or surrounding

ID: 263025 • Letter: I

Question

I a classical gonetic analysis, sciontists create mutations wthin or surrounding cloned gone sequences They than introduce these mutated gone sequences into col oultures or modal organisms and examine the phenatypes. Gonoscists are now adding RNA-induced gane silencing to their gono-analysis toolbox Part A Desaribe how you would uso RNA-induced gana siloncing to study the rolo of the Drosophia tra gono in sex dotormination. Using RNA-inducod slancing, one cculd spooficaly doleto the tra gono in constant gonosc backgrounds. Using RNA-inducod sonaing, ono cculd spoaficaly ator tra pre-mRNA lavols in constant genetio backgrounds tra pre- mRNA lavols Using RNA-nducod slancing, one could speoficaly induce mutatons in tra pre-mRNA in constant ganetic backgrounds Using RNA-nduced slencing, one could mutate the tra gene on each stage and study how the process would proceed Correct Part B Next, describe how you would study tra function, using a dassical genetic analysis Mutatons Mutaions in the tra gene can be isclated by screens to determine the action of tra on maleifemale diferentiaton Mutations in the ?e gene sequences should be just introduced into model organisms, and the phenotypes examined. Mulasons in the tre gene can be radioactvely labeled to study tra function in sex determination Mutaions in the tra gene can be identfled by sequencing to determine the action of tra on malefemale differentation. g to determine the Correct

Explanation / Answer

Answer:- The correct option for Part A and Part B is Option B. As i can see you have got the correctly.

The correct options for the third question are first three options.

RNA induced silencing is relatively specific.

The classical approach is laborious and influenced by genetic backgrounds of mutant strains.

Introduction of double stranded RNAs into cells in RNA incuced silencing produces an artificial environment in which to study gene action.