Proteins usually aren\'t that stable, apply heat or detergent and they unfold. O

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Proteins usually aren't that stable, apply heat or detergent and they unfold. Once they unfold, these proteins will form large aggregates (i.e., the protein molecules will clump together). A) Explain why this aggregation occurs once the protein unfolds. Include the interactions that occur to stabilize these aggregates. B) Propose a method for refolding these proteins. 1. 2. Describe the difference between a screen and a selection 3. Describe how DNA shuffling works. 4. How do you test individual members of a library such that you can isolatea 5. Draw out each of the 20 amino acid side chains 6. If an organism repurposed one of the degenerate codons to code for a 21st hit from the vast majority of non-improved and loss-of-activity mutants? amino acid, do you think this organism would have a fitness advantage over other organisms? Explain your logic. When designing a library, you need to be able to successfully test the entire synthesized library, having at least a 99% probability to have at least one sample of each theoretical member in your library. If you want to do a saturation library of 4 amino acid positions, how large is your theoretical diversity for this library? How many samples must you test to achieve this 99% probability? 7.

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