1. Case: Sloppy sandwich-making serves up squeamish stomach A young woman in her
ID: 261283 • Letter: 1
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1. Case: Sloppy sandwich-making serves up squeamish stomach A young woman in her 20s comes into your clinic with severe diarrhea, stomach cramps and fever. She tells you that she ate at a Subway sandwich shop for lunch the previous day and didn't feel well soon afterwards. You suspect that she has some kind of enteric pathogen and send off samples of her stool for various tests. What do you observe microscopically if indeed she has an enteric pathogen? (Gram reaction and cell morphology) What type of media could be used to isolate this type of bacteria? What can you conclude from the following biochemical tests? P-R lactose broth is a yellow color and Durham tube has 20% of its liquid displaced Tryptone broth has a pink ring on top after addition of indole reagent KIA slant is yellow throughout Based on these test results, what organism is causing her symptoms? How might this organism have been transmitted to her?Explanation / Answer
If it is enteric pathogen, then it must be gram negative bacteria. When I will see microscope, it will we have thin cell wall. When this bacteria undergo, gram staining result, then the bacteria will not retain the color of the crystal violet.
Mannitol salt sugar media will be used to isolate this type of bacteria .
Bio-chemical test will provide the information about the complete morphology of the pathogen.
The probable organism is salmonella typhii. This bacteria has been transmitted from the food that she has eaten.
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