You\'re a park ranger at Yellowstone National Park. You\'ve discovered the inter

Question

You're a park ranger at Yellowstone National Park. You've discovered the internal organs of a deer just inside the park boundaries. Because hunting in the park is illegal, you notify the local police to be on the alert for a recently killed deer. The police spot a truck carrying the carcass of a large deer a few miles outside the park. They suspect this is the deer that was killed inside the park boundaries, but they need somes evidence linking this deer to the remains found in the park.

Would test(s) could determine whether the deer in the truck was killed inside Yellowstone Park? Explain your answer.

Explanation / Answer

In order to determine the weather, the carcass belongs to the deer whose internal organs were found just inside the park boundaries we need to do DNA fingerprinting or Restriction Fragment Length Polymorphism (RFLP) of the organ found inside and the carcasses found in the truck.

First, we need to isolate the genomic DNA of the carcass and the internal organ. Each organism has different alleles for the same genes. This will give us the different bands on the agarose gel when we amplify the gene which is found in polymerism in the deer. If the bands of the carcass and the band of the internal organ migrate at the same point then we can say that the deer were killed inside the park.

In Restriction Fragment Length Polymorphism- In this technique, the DNA is digested by the restriction endonuclease. DNA present in each organism is different from each other due to polymorphism.So when we digest the DNA we will get different bands on the gel which is the pattern of each organism.

So when we digest the DNA of carcass and the internal organ we will get the same pattern if the carcass belongs to the same organism

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