Suppose a chemist synthesizes four new chemical compounds in the laboratory and

Question

Suppose a chemist synthesizes four new chemical compounds in the laboratory and names them PFI1 PF12, PF13, and PFI4. He gives the PFI compounds to a geneticist and asks her to determine their mutagenic potential. The geneticist finds that all four are highly mutagenic. She also tests the capacity of mutations produced by the PFl compounds to be reversed by other known mutagens, and obtains the results shown in the table below Mutations reversed by Mutations produced by 2-Aminopurine Nitrous acid Hydroxylamine Acridine orange PFI1 PF12 PF13 PFI4 Yes No Yes No Yes No Yes No Some No Yes Use the data in the table to match the chemical compounds PFI1, PF12, PFI3, and PF14 to the type of mutation that the compound is most likely to cause. X Transversions or large deletions Base pair transitions Single-nucleotide insertions or deletions PF12 PFI4 PFI1 PF13 Incorrect See lower panel for more information

Explanation / Answer

To answer this question first let us try to understand the mutagenic affect of the reversion agents used.

2-Aminopurine

Nitrous acid

Hydroxylamine

Acridine orange

GC to AT and AT to GC transitions

GC to AT and AT to GC transitions

GC to AT transitions

Single-base insertions or deletions resulting in frame shift mutations.

Mutations caused by PFI1 can be reversed by 2-Aminopurine, Nitrous Acid and Occasionally by Hydroxylamine, it can be suggested that PFI1 causes both types of transitions GC to AT and AT to GC.

Mutations caused by PFI2 are not reversed by any reversion agents, it can be inferred that PFI2 causes transversions of large deletions.

Mutations caused by PFI3 can only be reversed by 2-Aminopurine and Nitrous Acid it suggests that it causes GC to AT transitions.

Mutations caused by PFI4 are reverted only by Acridine Orange, it suggested that it causes single-base insertions or deletions

2-Aminopurine

Nitrous acid

Hydroxylamine

Acridine orange

GC to AT and AT to GC transitions

GC to AT and AT to GC transitions

GC to AT transitions

Single-base insertions or deletions resulting in frame shift mutations.

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