4. Set1 is a histone methyltransferase that specifically methylates histone H3 a

Question

4. Set1 is a histone methyltransferase that specifically methylates histone H3 at the lysyl residue located at the fourth position (H3K4) within the exposed tail. Set1 can mono-, di-, or trimethylate H3K4 and this degree of methylation can impact nucleosome positioning and thus gene expression levels. a. SET1 is the gene that encodes Set1. It is being cloned into the pRS406 vector shown below: Created with SnapGene (4319) AatIl Pfol (46) 4317) Zral Ndel (330 BsgI (363) (3996) Xmnl SbfI (399) BRAI . BspM1 (402) XcmI (545) PpuM! ?570) Ncol (622) BstBI (682) BbsI (691) BsmI (779) (3545) NmeAIII StuI (853) AmpR Proe (3397) AhdI PRS406 4384 bp BpuioI (147) BfrBI (1242) MCS 13 rev NsiI (1244) BsaAl (1575) BtgzI (1576) NgoMIV (1676) ac promoter Nael (1678) Acc65I (2004) KpnI (2008) Abs1 . PaeR71 . Pspx1 . T111 . Xhol (2019) BspDI-Clal (2035) Hindill (2040) EcoRI C2052 TspMI XmaI (2064) Smal (2066) BamMI (2070) Spel (2076) Xbal (2082) EagI Noti (2089) Alel (2100) SacII (2101) BstXI (2102) Ecos3ki (2108) SacI (2110)

Explanation / Answer

1. the multiple cloning site is specifically present in the lac Za region of the plasmid. This is particularly useful for the blue white selection process. It will be grown in the media containing X gal + IPTG.

2. set1 .

3. Xho1 is present at 2019 bp and Sac II is present at 2101 bp. This removes an unit of 82bp. Hence, the new linearised plasmid is of 4382bp.

Related Questions

Navigate

• Browse (All)
• Browse 4
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.