1. A scientist wants to understand Gene X, which is expressed in response to adr
ID: 259273 • Letter: 1
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1. A scientist wants to understand Gene X, which is expressed in response to adrenalin in mammals a) (5 pts) Which of the following techniques or resources could be used to determine the size of the mature transcript of Gene X in different tissues? (circle all correct possibilities) a genomic library a cDNA library PCR-amplification of genomic DNA PCR-amplification of cDNA southern blot northern blot b) (5 pts) Using one of the correct techniques above, she finds that the transcript is 800 bases long in lung tissue, and 1200 bases in muscle tissue which of the following could be true? A. the mature muscle transcript includes additional introns B. the mature muscle transcript contains different exons than the mature lung transcript C. there is a premature stop codon in the lung transcript that stops transcription early D. the 5' cap is longer in the muscle tissue c) (5 pts) Later, she found that a point mutation destroys the splice site at the 5' end of an intron in Gene X, and that splice site 6 bases downstream is used instead. Which of the following could be true? A. The mRNA of the mutant will encode the exact same protein as the normal mRNA B. The extra 6 bases will cause a frameshift, leading to disruption of the protein downstream C. Two additional amino acids will be added to the protein produced from this mRNA D. This mutation will cause the intron to be included in the mature transcriptExplanation / Answer
1(a)the correct option to find the size is PCR amplification of cDNA which will first result in the formation of RNA from that using reverse transcription you can preapre cDNA by amplification we can compare the size which will be equal to the size of the RNA transcript ..
(b) the correct option is option (b) the difference in the size of the exons present in them because as it mature transcript no introns will be present due to splicing,,, Secondly in transcription there is no use of the stop codon..
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