1. A scientist wants to understand Gene X, which is expressed in response to adr
ID: 257400 • Letter: 1
Question
1. A scientist wants to understand Gene X, which is expressed in response to adrenalin in mammals. a) (5 pts) Which of the following techniques or resources could be used to determine the size of the mature transcript of Gene X in different tissues? (circle all correct possibilities) a genomic library a cDNA library PCR-amplification of genomic DNA PCR-amplification of cDNA southern blot northern blot b) (5 pts) Using one of the correct techniques above, she finds that the transcript is 800 bases long in lung tissue, and 1200 bases in muscle tissue which of the following could be true? A. the mature muscle transcript includes additional introns B. the mature muscle transcript contains different exons than the mature lung transcript C. there is a premature stop codon in the lung transcript that stops transcription early D. the 5' cap is longer in the muscle tissue c) (5 pts) Later, she found that a point mutation destroys the splice site at the 5' end of an intron in Gene X, and that splice site 6 bases downstream is used instead. Which of the following could be true? A. The mRNA of the mutant will encode the exact same protein as the normal mRNA B. The extra 6 bases will cause a frameshift, leading to disruption of the protein downstream C. Two additional amino acids will be added to the protein produced from this mRNA D. This mutation will cause the intron to be included in the mature transcriptExplanation / Answer
Answer 1a) a cDNA library and PCR amplification of cDNA as its a mature transcript that means RNA and this data will be available in cDNA library and for determining the size of transcript she use PCR to amplify and through she can sequence the RNA.
B) the mature muscle transcript contains different exon than the mature lung transcript because there might be different exon of same gene for the stability of protein.
C) This mutation will cause the intron to be included in the mature transcript as there is addition of 6 bases which causes increase in intron size which interrupt the splicing. These bases come in the mature transcript.
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