Biol101 Activity 3: Below is the cDNA for the human protein PHF5A. The protein s

Question

Biol101 Activity 3: Below is the cDNA for the human protein PHF5A. The protein sequence is indicated below their respective codons: 1 aggtcggacggaagttcccgaagcttccggtggccggcttagttaggagctatggctaaa MetAlaLys 61 catcatcctgatttgatcttttgccgcaagcaggctggtgttgccatcggaagactgtgt HisHisProAspLeuIlePheCysArgLysGlnAlaGlyValalalleGlyArgLeucys 121 gaaaaatgtgatggcaagtgtgtgatttgtgactcctatgtgcgtccctgcactctggtg GluLysCysAspGlyLysCysValileCysAspSerTyrValArgProcysThrLeuVal 181 cgcatatgtgatgagtgtaactatggatcttaccaggggcgctgtgtgatctgtggagga ArgileCysAspGlucysAsnTyrGlySerTyrGlnGlyArgCysValllecysGlyGly 241 cctggggtctctgatgcctattattgtaaggagtgcaccatccaggagaaggacagagat ProGlyValserAspAlaTyr TyrCysLysGlucysThrileGlnGluLysAspArgAsp 301 ggctgcccaaagattgtcaatctggggagctctaagacagacctcttctatgaacgcaaa GlycysProlysileValAsnLeuGlySer SerLysThrAspLeuPheTyrGluArgLys 361 aaatacggcttcaagaagaggtgattggtgggtggccccttcctccccccaacatcagtc LysTyrGlyPhelysLysArgend

Explanation / Answer

1.

Forward: 5'-atggctaaacatcat-3'

Reverse: 3'-aagttcttctccact-5'

2.

Forward: 5'-gaattcatggctaaa-3'

Reverse: 3'-aagttcttctccact-5'

3.

Forward: 5'-atggctaaacatcat-3'

Reverse: 3'-ttctccactcttaag-5'

4.

Forward: 5'-atggctaaacatcat-3'

Reverse: 3'-ttcacacactaaaca-5'

5.

Forward: 5'-tgtgactcctatgtg-3'

Reverse: 3'-aagttcttctccact-5'

6.

Forward: 5'-atggctaaacatcat-3'

Reverse: 3'-ttcacacactaaCCA-5'

7.

Forward: 5'-GGTgactcctatgtg-3'

Reverse: 3'-aagttcttctccact-5'

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