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Biochemistry: Finding the dye concentration (µg/mL). Three dilutions were made u

ID: 199493 • Letter: B

Question

Biochemistry: Finding the dye concentration (µg/mL).
Three dilutions were made using a stock solution of 150 µg/mL and water. For the first dilution, 2 µL of stock was diluted with 3 mL of water. For the second dilution, 5 µL of stock was diluted with 3 mL of water. For the third dilution,10 µL of stock was diluted with 3 mL of water.
Find the dye contration after each dilution. Answer in µg/mL. Please show work, I do not know how to approach the problem. Biochemistry: Finding the dye concentration (µg/mL).
Three dilutions were made using a stock solution of 150 µg/mL and water. For the first dilution, 2 µL of stock was diluted with 3 mL of water. For the second dilution, 5 µL of stock was diluted with 3 mL of water. For the third dilution,10 µL of stock was diluted with 3 mL of water.
Find the dye contration after each dilution. Answer in µg/mL. Please show work, I do not know how to approach the problem.
Three dilutions were made using a stock solution of 150 µg/mL and water. For the first dilution, 2 µL of stock was diluted with 3 mL of water. For the second dilution, 5 µL of stock was diluted with 3 mL of water. For the third dilution,10 µL of stock was diluted with 3 mL of water.
Find the dye contration after each dilution. Answer in µg/mL. Please show work, I do not know how to approach the problem. Three dilutions were made using a stock solution of 150 µg/mL and water. For the first dilution, 2 µL of stock was diluted with 3 mL of water. For the second dilution, 5 µL of stock was diluted with 3 mL of water. For the third dilution,10 µL of stock was diluted with 3 mL of water.
Find the dye contration after each dilution. Answer in µg/mL. Please show work, I do not know how to approach the problem.

Explanation / Answer

Answer

1. For the first dilution, 2 µL of stock was diluted with 3 mL of water.

      In this 2 µL added in 2998 µL Water

                   2/3000 = 1/1500 = 1:1500 times dilution.

2. For the second dilution, 5 µL of stock was diluted with 3 mL of water.

In this 5 µL added in 2995 µL Water

                   5/3000 = 1/600 = 1:600 times dilution.

3. For the third dilution,10 µL of stock was diluted with 3 mL of water.

In this 10 µL added in 2990 µL Water

                   10/3000 = 1/300 = 1:300 times dilution.