4) Westem blot: In a westem blot, what substance has an enzyme attached to it, s

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4) Westem blot: In a westem blot, what substance has an enzyme attached to it, so you can detect where a primary antibody has bound? Secandar ant body a) /b) What is the name of something that an antibody binds to or rec 5) Ralph is performing a westem blocking solution, and then adds the secondary antibody. He then rea antibody, so he adds it after the secondary antibody blot, and staining for the protein actin on a membrane. He adds the lizes he forgot to add a primary a) What is a primary antibody? An antibody thet binds b) Why will adding a primary the second ects to a protein antibody after the secondary antibody not work? Explain what cannot bind? Aprimar the secondary antsbody is in ts placg

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