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1. Which one of the following scientists helped to demonstrate that DNA, not pro

ID: 25833 • Letter: 1

Question

1. Which one of the following scientists helped to demonstrate that DNA, not protein, was the genetic information that viruses inject into bacteria?

Alfred Hershey
Rosalind Franklin
Colin MacLeod
Fredrick Griffith
Maclyn McCarty

2.Which one of the following structures is most accurately described as euchromatin?

700-nm fibers
300-nm fibers
10-nm fibers
A purified 2-nm DNA double helix
A condensed mitotic chromosome

3. Which one of the following statements about transcription is correct?

RNA polymerase begins to unwind the DNA near the -10 site.
The E. coli RNA polymerase holoenzyme consists exclusively of two ? subunits, one ? subunit, one ?' subunit, and an ? subunit.
In E. coli, the -10 site for "gene X" is likely to be very different than the -10 site for "gene Y."
? factor is always necessary for RNA polymerase to transcribe the next base.
Rho protein assists in termination of transcription for all E. coli genes.

4. dentify if each of the following statements about the discovery of DNA structure is True or False.

One of the findings of Franklin and Wilkins X-ray crystallization experiments was that DNA is double stranded.
One of the findings of Franklin and Wilkins X-ray crystallization experiments was that DNA has a 2 nm uniform width that is helical.
Chargaff's rules include that the ratio of pyrimidines to purines is 1:1.
Avery, MacLeod and McCarty identified Griffith's transforming principle as a virus.

5. During DNA replication, which one of the following enzymes is responsible for preventing supercoiling?

Topoisomerase
Kinase
Primase
DNA polymerase I
DNA polymerase III

6. E. coli RNA Polymerase:

copies the information in DNA into RNA.
consists of 6 subunits, collectively referred to as the Core Enzyme.
consists of 7 different polypeptide chains.
requires dNTPs.
copies the information in RNA into RNA.

7. The role of primase during DNA replication is to:

remove RNA nucleotides from newly-synthesized DNA.
synthesize short RNA fragments that serve as "primers" for DNA synthesis.
synthesize DNA in the 3' to 5' direction away from the ori.
use an RNA template to add the first 3-10 nucleotides of a new DNA strand.
synthesize the Okazaki fragments on the lagging strand of DNA synthesis.

8. One of the functions of a telomeric region of a chromosome is to:

hold sister chromatids together throughout the cell cycle.
help separate the sister chromatids during mitosis.
allow for accurate replication of the ends of linear chromosomes.
accurately replicate circular chromosomes.
None of the above accurately describe the function of telomeres.

9. Match the molecule or complex with the appropriate stage of transcription.

A Initiation
B Promoter recognition
C Termination
D Elongation

Rho
Closed complex
RNA polymerase core enzyme
Open complex
Sigma factor

10. The strands that make up DNA are antiparallel. This means that:

purines bond with purines and pyrimidines bond with pyrimidines.
the 5' to 3' direction of one strand is counter to the 5' to 3' direction of the other strand.
the twisting of the DNA molecule has shifted the two strands.
the base pairings create unequal spacing between the two DNA strands.
one strand is positively charged and the other is negatively charged.

11. Identify whether each of the following statements about DNA replication is True or False.

DNA polymerase III is the major DNA synthesizing enzyme in E. coli.
DNA polymerases require a free 3' phosphate group to which an incoming dNTP is added.
When DNA polymerase III begins synthesis, it covalently links a DNA base to a DNA base.

12. Which of the following sequences would function as the strongest -10 site in E. coli?

5

Explanation / Answer

1.C 2.D 3.C 4.A 5.D 6.B 7.A 8.A 9.B 10.C 11.D 12.A 13.B 14.B 15.B 16.C 17.C