2. DNA Synthesis a. You decide for fun that you want to repeat the Meselson/Stah
ID: 257422 • Letter: 2
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2. DNA Synthesis a. You decide for fun that you want to repeat the Meselson/Stahl experiment. You grow your cells first in media containing radioactive nitrogen and then shift to non-radioactive media at t-0. t 0 1st round 2nd round i. Which of the following best describes the expected results after 1 round of replication? A. Identical result as the t 0 lane. B. The same band as the t-0 lane plus a second band lower on the Y axis C. The same band as the t-0 lane plus a second band higher on the Y axis. D. A single band lower on the Y axis compared to the t 0 band. ii. Which of the following best describes the expected results after 2 rounds of replication? A. The same band as the t 0 lane plus a second band lower on the Y axis. B. A single band lower on the Y axis compared to the t-0 band. C. Two bands, both that are lower on the Y axis compared to the t-0 band D. Two bands, both that are higher on the Y axis compared to the t-0 band b. True or False. In theory, it is possible for an RNA strand to consist of nucleotides connected by 2'-5' phosphodiester bonds instead of 3'-5' bonds. Which of the statements below best explains why you selected true or false? A. Phosphodiester bonds can only be formed between 3 and 5' carbons B. The uracil base allows for increased reactivity compared to the thymine base. C. RNA has an OH group on the 2' carbon. D. DNA is more stable than RNA c. In some cases, replication is not bi-directional and a single helicase opens the DNA in only one direction. How would this affect the mechanism of DNA replication in terms of leading and lagging strand synthesis for prokaryotic cells? Select the best answer. A. It would not affect the mechanism. B. It would eliminate the need for DNA ligase C. It would mean that there would only be leading strands, not lagging strands. D. It would eliminate the need for telomerase.Explanation / Answer
answer ai - option c is correct radioactive strands will increase with each round of replication
answer aii- option d is the correct reason is stated above
answer b A- TRUE. the phosphodiester bond is the bond between the 3' C atom of one sugar molecule and the 5' C atom of another.
answer b B- TRUE. thymine is more stable due to the methyl group
answer b C- FALSE. Ribose in RNA has 2'OH group which make it highly reactive
answer b D- TRUE- due to presence of deoxyribose sugar and thymine base it is more stable than RNA
answer B- FALSE. OPTION A is correct , reason stated above
answer C- answer a is correct. rest all options are wrong because it do require telomerase, ligase, and have lagging strands as well.
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