76. A single-pass transmembrane protein is destined to be transported to the pla

Question

76. A single-pass transmembrane protein is destined to be transported to the plasma membrane. In the wild-type protein, a lysine flanks the transmembrane domain on the N terminal side, and a glutamic acid flanks on the C-terminal side: Both the N- & C-terminal soluble domains carry sequences that can by glycosylated by oligosaccharyltransferase. A. (1pt) After the protein is transported to the plasma membrane, will the N-or the C- terminal soluble domain be on the extracellular side? B. (1pt) Is the glycosylation added in the ER onto the N- or the C-terminal soluble domain?

Explanation / Answer

Usually C terminal soluble domain will be in extra-cellular space since most of surface protein carries a negative charge, and C terminal contains a glutamic acid residue that can be easily converted to negative charge -COO- . Glycosylation would take place on C terminal soluble domain.

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