BIOS 221 ene Expression Worksheet Real-time PCR Questions 10 0 2 4 6 8 10 12 14

Question

BIOS 221 ene Expression Worksheet Real-time PCR Questions 10 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Cycle 15. Assuming the fluorescence threshold is set to be 1. Which gene has the highest gene expression rate? What is the Ct value? Gene with highest expression- 16. Which gene has the lowest gene expression rate? What is the Ct value? Gene with lowest expression = Ct value - 17. Assuming real-time PCR was conducted on Gene 8, not shown on the graph above. If Gene 8 has a Ct value of 10, how would you interpret the result?

Explanation / Answer

15. gene with higher expression= gene 1

ct value=14

16. gene with lowest expression= gene 7

ct value= 22

17. If gene 8 has the ct value of 10, the gene 10 will be the highly expressive of all the 8 gnees shown in the graph.

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