2- A molecule of DNA was digested with two resirie separate reaction, named enzy

Question

2- A molecule of DNA was digested with two resirie separate reaction, named enzyme 1 and enryr The order of loading samples are as follow the size of bands) ually, tone enayme ose wet The samples were ran 30 Points) Use graph and show how you estimate I:uncut DNA 2: cut DNA with restriction enzyme number 1 3: cut DNA with restriction enzyme number 2 15000 bp, 400 bp and 70 bp 4:DNA maker, Answer the following This DNA contained how many restriction site for enzyme 1 and enzyme 2 containing 5 DNA fr 15 Kbp, agments as follow 35 K bP, question This molecule of DNA belong to prokaryotes or Eukaryotes, why What are the size of DNA fragments cut by enzyme 2 1 2 3 4 S, oo 400 70 35

Explanation / Answer

1. Enzyme linearizes the plasmid. So, it must contain 1 site for enzyme 1. Enzyme 2 produces 3 bands. So, it must contain 3 sites for enzyme 2.
2. The DNA could be from eukaryotes. This is because most of the restriction enzymes are isolated from prokaryotes which do not digest their own DNA.
3. Fragments produced by enzyme 2 digestion = 50 bp + 200 bp + 7,500 bp

For question 3, the marker sizes are given incorrectly. The bands are not shown to be running at the proportionate logarithmic scale.

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