2- A molecule of DNA was digested with two restriction enzymes individually, (on

Question

2- A molecule of DNA was digested with two restriction enzymes individually, (one enzyme in each The samples were ran on an agarose gel. The order (30 points) separate reaction, named enzyme 1 and enzyme 2, of loading samples is as follow :uncut DNA 2: cut DNA with restriction enzyme number 1 3: cut DNA with restriction enzyme number 2 4:DNA maker, containing 5 DNA fragments as follow 35 K bPp, 15 Kbp, 1500 bp, 400 bp and 70 bp Answer the following question This DNA contained how many restriction sites for enzyme 1 and enzyme 2? This molecule of DNA belong to prokaryotes or Eukaryotes Caiculate the size of DNA fragments cut by enzyme 2, compare to the total size of uncut DNA

Explanation / Answer

A) There is only one site for restriction enzyme 1 and there are 3 site for restriction enzyme 2.

B) The DNA is from prokaryotes because The DNA is circular and we can say that from the band pattern of restriction enzyme 1 because there only one band even if the site for restriction enzyme is present.

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