would you solve six questions? Food Testing Assignment 3M Plate Use and Interpre

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would you solve six questions?

Food Testing Assignment 3M Plate Use and Interpretation Matrix Type of plate mL of Top film,Type of Incubation Id sample toDrop it or spreader time at 35 colony by use; 1 or 3? Roll it small or degrees C? colony down?large?", flat color and or ridge side down? small, ridge 48 hours side down small, flat 24 hours side down features? Aerobic Plate drop Coliform (total)* 1 red and blue with roll 9 as small, flat24-48 hours side down large Escherichia coli* 1 roll blue with gas Staph Express 1 Plate Enterobacteriaceae 1 Plate 24 +- 2 hours red-violet small, flat 24 +/-2 hours red with side down roll gas and/or yellow acid zone nearby 3 Environmental Listeria Plate 28 +- 2 hours, intense not over 30 red-violet roll large 5 - located on same type of plateIhours * All spreaders are relatively small, except for Staph Express & Listeria Food Testing Results Food AerobicColiformE.coliStaph Enterobacter Listeria Fruit mix Raspberries Spinach Cheese Mix

Explanation / Answer

1. Types of food borne pathogens in Enterobacteriaceae petri-film plate media:

The petri film indicated acid production. Genus belonging to enterobacteriaceae: Klebsiella, Enterobacter Escherichia are lactose fermenting, others are variable or slow acid producers.

Answer:

Klebsiella, Enterobacter, Escherichia, Salmonella, Shigella, Yersinia.

2. Three plates that identifies genus causing food borne illness are:

Environmental Listeria plate: indicate failure in handling food sample and risk but may not reflect immediate food borne hazard.

Answer:

a. Escherichia coli, b. Staphylococcus express plate, c. Enterobacteriaceae plate

3. Plates that identifies genus causing food borne infection:

Enterobacteriaceae plate may or may not have infective pathogen like Salmonella, Shigella, or pathogenic E. coli.

Similarly, E. coli plate may not have pathogenic strain.

Thus, infective dose of Listeria may be related to food borne infection.

Answer: Environmental Listeria plate

4. Plates that identifies genus causing food borne intoxication:

Food containing toxins (exotoxins) produced by microorganisms, causes food borne intoxication.

Answer:

Staphylococcus express plate.

Table is not filled with data for each food, for to answering other parts.

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