Pre-Lab Report Sheet Enzyme Activity Lab Lab Section Name: Before coming to labs

Question

Pre-Lab Report Sheet Enzyme Activity Lab Lab Section Name: Before coming to labs carefully read the previous pages on Enzyme Activity then answer these pre-lab questions. Be prepared to hand in your responses to the pre-lab questions at the start of lab. 1. Use your knowledge of chemistry and that of enzyme activity in cells gained by reading the perspectives section to this lab experiment to explain why the rate of enzyme activity fastt at the start of an enzyme catalyzed reaction and gradually declines as the reaction proceeus 2. Under what conditions would the rate of enzyme activity (e.g. catalase's activity) in a cell remain constant and not decline? Explain. 3. Read the procedures for Parts A, B, & C and write a hypothesis for each section then summarize (part A completed) what will be done in each part in the spaces below. Part A. Effect of Enzyme Concentration on Enzyme Function Hypothesis:

Explanation / Answer

1. the rate of enzyme activity is the fastest at the start of an enzyme-catalyzed reaction Because there are higher substrates for the enzyme to function on in the starting.the reactions are random chance reactions which rely on the physical interaction between the enzyme and the substrate, this occurs at a higher rate at the beginning of the reaction.

2.  The enzyme work as long as the substrate is available. If the concentration of substrate and enzyme are constant, the enzyme activity will also be constant. Enzymes are influenced by environmental conditions like pH, temperature and ionic concentration etc. therefore environmental conditions must also be constant. the enzyme should also be a perfect enzyme meaning that it cannot be saturated by the substrate.  

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