13. Assume that homologous recombination occurs across a region of DNA containin

Question

13. Assume that homologous recombination occurs across a region of DNA containing a wild-type and mutant allele of a particular gene. There is a single base pair change in the mutant allele as follows:

wild-type = 5’…TTGGGTA… à5’…TTGCGTA… = mutant allele, such that the resulting dsDNA is as follows:

     5’…TTGGGTA…

     3’…AACGCAT…

What would be the genotype (wild-type or mutant) if base excision repair occurs on the TOP strand?

14. What term describes the process occurring above?

15. Sensors in the nucleus are able to detect and repair double strand breaks in the DNA. Briefly (one sentence please) explain why these sensors do not detect the ends of eukaryotic chromosomes and repair these ends by linking different chromosomes together?

Explanation / Answer

13) the genotype will be mutant. As the non template strand is the top one, the ptotein that will be produced will be from the mutant allele.

14) the process above is known as base excision repair

15) the ends of a eukaryortic chromosome contains repetitive DNA segments and is called a telomere. This region is bound by a protein complex called shelterin, which protects it from non-homologus end joining or homologus end joining.

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.