5. Oxidative phosphorylation in mitochondria is often monitored by meas uring ox

Question

5. Oxidative phosphorylation in mitochondria is often monitored by meas uring oxygen consumption. e oxygen. If there is little ox. When ox. phos. Is proceeding rapidly, the mito. will rapidly consum phos., only small amounts of oxygen will be used. You are given a suspension of mitochondria and directed to add the following compounds in the order from a to h addition of each compound, all of the effect of each addition on oxygen consumption by the isolated mitochondria . With the ed compounds remain present. Predict the . (8 pts) a) Glucose b) ADP+P c) Citrate d) Oligomycin e) Succinate f) 2,4-Dinitrophenol 8) Rotenone (inhibits Cmplx I) h) Cyanide

Explanation / Answer

5.

a) Glucose: Oxygen consumption will increase
b) ADP +Pi: Oxygen consumption will increase
c) Citrate: Oxygen consumption will increase
d) Oligomycin: Oxygen consumption stops
e) Succinate: Oxygen consumption will increase
f) 2,4- Dinitrophenol: Oxygen consumption will decrease
g) Rotenone ( inhibits complex I): Oxygen consumption stops
h) Cyanide: Oxygen consumption stops

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