B5. CCC TAGCTTTCCC T G AAACAT ACATA TGGTGTT TIT AC T 460 470 480 490 The above r
ID: 254185 • Letter: B
Question
B5. CCC TAGCTTTCCC T G AAACAT ACATA TGGTGTT TIT AC T 460 470 480 490 The above represents a sequencing "trace" that resulted from a sequencing reaction using a single- stranded template. The numbers above the trace are the distance of that peak from the primer (so, we are seeing just part of the whole sequence). In this trace, C's are blue, T's are red, A's are green and G's are black. Is the DNA polymerase moving left to right or right to left on this template? Looking at the sequencing trace above, we can tell that the first few nucleotides of the TEMPLATE were (please circle one of these): 5 CCCTAG 3 CCCTAG 5GGGATC 3'GGGATC 3' AGTAAA What would the trace look like if the person doing the reaction forgot to put in the green nucleotide? Please explain your answer below, and indicate the resulting changes on the sequence above.Explanation / Answer
DNA polymerase will move from right to left.
Possible primer sequence are: 5’ CCCTAG and 5’GGGATC as DNA polymerase synthesize 3’ to 5 ‘direction and primer are synthesized in opposite direction.
If the person, forget to put green nucleotide then as green peaks are representing Adenine residue, so will not be able to see adenine residue in trace peaks. The resulting trace will be have color peak of blue, red and black, but green will be missing.
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