11. (1pt) What is the normal cellular function of GM130? marks the Golgi in the
ID: 253550 • Letter: 1
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11. (1pt) What is the normal cellular function of GM130? marks the Golgi in the cell. It acts as a tether for vesicular transport to the Golgi c. It helps CMV to bud from the Golgi d. It helps to traffic US3 to the Golgi e. It helps to traffic PDI to the ER. 12. (2pt) Where do the mutants localize compared to wildtype (normal) US3? 13. (1.5pt) What is Brefeldin A? the endadasmic re 14. (1.5pts) What would happen to sub-cellular localization of each of the constructs in Figure 4 if the cells were treated with Brefeldin A (include the WT US3 protein in your answer)?Explanation / Answer
11) GOLGA2 or GM130 is a cis-Golgi matrix protein that have a rich coiled-coil structure & is strongly attached to the Golgi membrane. It acts as a vesicle tether which facilitates vesicular transport to the Golgi membrane. Apart from this it also plays a major role in mitotic Golgi disassembly & involved in the Golgi assembly maintenance. So, the correct option will be b.
13) Brefeldin A is a fungal metabolite produced by fungus Eupenicillium brefeldianum. Its found as white to off-white crystalline solid which is soluble in acetone, methanol, ethanol, DMSO etc. It inhibits the protein transport from endoplasmic reticulum (ER) to Golgi apparatus by uncompetitively binding to the GBF1 (Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1), which ultimately prevents the coat protein association with Golgi membrane.
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