chromosome number 4 are bases and 2\' bases \' \' ? the forward primer is 53 bas
ID: 253545 • Letter: C
Question
chromosome number 4 are bases and 2' bases ' ' ? the forward primer is 53 bases away from the first repeat. The 3' base of the reverse primer is 28 bases away from the first repeat in that direction. Each repeat unit is 60 bases in length. A person has, on one of their number 4 chromosomes, 6 repeats. How long (in bp) should its PCR product be? Draw a picture mapping this region of chromosome 4, the placement of the primers and the length of the PCR product. 2. Even with compelling DNA fingerprint evidence to the contrary, suspects are sometimes found "not guilty." What problems with this type of evidence can arise and create doubt about the guilt of a person? 3. Briefly describe a microarray experiment of your own design. You can either compare transcriptional difference between two different cell types, or you can comp transcriptional differences that result from different conditions on a single cell type.V ld uou ha Inokina for in the microarray? (Your design should not be siniExplanation / Answer
1)
The forward primer is 20 bases long and is 53 bases away from the repeat.
there are 6 repeats of 60 base in length
the reverse primer is 21 bases and is 28 bases from the repeat.
the length of the PCR product is 20 + 53 + (60*6) + 28 + 21= 482 bases
the map looks as follows
Fwd primer spacer repeat * 6 spacer rv primer
20b 53b 60 * 6 = 360 28b. 21b
5'_______------------repeat-repeat-repeat-repeat-repeat-repeat------------_______3'
2) If a suspect’s DNA is found at the scene of a crime, it proves that the DNA of the suspect was left there and not the presence of the suspect during the time of the crime.
DNA can be moved very easily from one place to another, whether by accident or intentionally.? Hence DNA can be transferred from one location to another and deposited on an object (crime scene evidence) very easily.
Therfore due this possibility, even in presence of compelling DNA evidence, most of the time suspect are found not guilty.
3) The following is a microarray experiment designed to study the genes that are altered when E. coli is grown in LB media at two different temperatures, say 37 C and 42 C.
- First a primary culture is made and grown overnight at 37 C
- This primary culture is used innoculate 2 different flasks containg LB. One flask is incubated at 37 C and the other at 42 C
- Both cultures are grown till they reach an OD of 0.8
- Once this OD has been reached, cells are pelleted RNA isolated
- the RNA is procesed t oget cDNA and microarray is performed.
- The data that is obtained is mapped to referecne genome and the genes that are upregulated and downregulted can be read as fold change in transcript levels.
this experiment is usually repeated in triplicated to determine siginifiace of the result
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