Exercise 3. Respiration in dry vs germinating peas eeds represent a dormant stag

Question

Exercise 3. Respiration in dry vs germinating peas eeds represent a dormant stage of a plants life cycle. Germinating peas, much like animal embryos, are going through massive amounts of cell division and therefore require huge amounts of energy to develop and grow. A germination pea should exhibit notable amounts of cellular respiration which we can observe again using a pH indicator, meth Obtain four large test tubes and label t 1. two-thirds full of germinating peas 2. two-thirds full of germinating peas ce the following in each: 3. two-thirds full of dry heat-killed peas (to stop any minute amounts of respiration that may occur) 4. two-thirds full of plastic beads (why?) Into each place a small test tube containing methyl red, an indicator which turns from vellow to red in the presence of acid. Place parafilm over the lids of all 4 tubes and wrap tube 2 in aluminum foil so no light may enter. Let sit for 45 minutes and record any color change observed. Record/draw and explain the results in each tube. peo peus tn lud peas he in Note: It may seem annoying that we use two different indicators to look for pH changes, this is because phe red will not work in the system we have prepared for exercise 3.** Anaerobic Respiration/fermentation in yeast We will investigate anaerobic respiration using a yeast (Saccharomyces cerevisiae) photosynthetic arbohydrates such as sugars to carbon dioxide and alcohol. Yeast are facultative anaerobes w means that they can respire in the presence of oxygen but will switc gle-celled organism from the Fungus Kingdom (Division ascomycota). Yeast are r rather they are chemo-organotrophs that acquire energy through conver h to fermentation in its absen Exercise 4. Effect of temperature on fermentation Around the room you will find sugar solutions (either 1% glucose or sucrose), specifically marke cooling or heating to the temperature treatment indicated (4 for this experiment). (One bottle w on ice at O°C. One will be found at room temperature (-20 °C), one will be in the incubator at nd one will be heated to above h0e (but not beyond 80 °C) prior to putting yeast in the sol

Explanation / Answer

Tube1: Change of color from red to yellow is seen in the test tube-1because germinating seeds undergo respiration and causes liberation of carbon dioxide which turns methyl red to yellow color.

Tube 2: No change of color from red to yellow is observed in the test tube-2 due to lack of carbon dioxide because inhibition of photorespiration.

Tube 3: No change of color from red to yellow is observed in the test tube-3 due to inactivation of enzymes responsible for respiration in germinating seeds due to high temperature.

Tube 4: No change of color from red to yellow is observed in the test tube-4 due to non-living plastic beads.

Related Questions

Navigate

• Browse (All)
• Browse E
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.