1) Which one of the following best names the technique used in this experiment?
ID: 252166 • Letter: 1
Question
1) Which one of the following best names the technique used in this experiment?
A Western Blot
B DNA Footprinting
C Electrophoretic Mobility Shift Assay (EMSA)
D Sanger Sequencing
E Northern Blot
2) Complex 2 seen in lane 6 is most likely the protein _________ bound to the radiolabeled DNA sequence.
A TFIIA
B RNA polymerase II
C TFIID
D TFIIB
E TFIIE
3) Which one of the following is a correct statement about these experiments with DNA and trans acting proteins?
A The proteins are synthesized at the site where the proteins bind
B The cold DNA and the hot DNA combine to make binding just right.
C Some of the unlabeled DNA contains an AT rich area that binds less tightly (two hydrogen bonds instead of three hydrogen bonds)
D The proteins bound to the radioactive DNA are degraded by the emitted high energy particles so there is no more protein to bind to the unlabeled DNA.
E Some proteins in the transcriptional apparatus do not recognize and/or bind to the promoter sequence.
1-3. Passage 11-Transcription Factors -Questions 1-3 An experiment was designed to determine the sequential order in which transcription factors add to the initiation complex. In different combinations, the transcription factors were added to radiolabeled (hot) DNA containing the promoter sequence and promoter proximal sequences, followed by electrophoresis on a native gel (no denaturation) and autoradiography Seven different complexes of DNA and protein were observed (numbered 1-7 on the left side of Figure 1). None of the complexes are formed when the same experiments are performed in the presence of excess amounts of unlabeled (cold) DNA sequences that include a TATAAA sequence. Complexes 2-7 still form in the presence of cold DNA sequences when the cold DNA TATAAA sequence is mutated to TAGAGAA Complex 1 still does not form when the cold DNA sequence is TAGAGAA. The data for the competition experiments with cold DNA are not shown. TFI IA FIIB TFIID TFIIE RNA Po 1I complex 7 free probe 1 23 4 5 6 7 8 9 10 1 12Explanation / Answer
Answer:
1) Which one of the following best names the technique used in this experiment?
2) Complex 2 seen in lane 6 is most likely the protein _________ bound to the radiolabeled DNA sequence.
A TFIIA
B RNA polymerase II
C TFIID
D TFIIB
E TFIIE
3) Which one of the following is a correct statement about these experiments with DNA and trans acting proteins?
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