You have 20 ml of a liquid with 5.4 x 10^ 9 organisms/ml. You need exactly 3 x 1

Question

You have 20 ml of a liquid with 5.4 x 10^ 9 organisms/ml. You need exactly 3 x 10^2 organisms/ml in at least 25 ml but you have only the following dilutions and pipets. You must use the dilutions as is (no removing or adding volumes) and you can only use the pipets once. The pipets are only calibrated to the volume specified i.e. they are not graduated.

Supplies:
Pipets Dilutions Blanks
1 - .1ml 1 - 99.9ml 2 - 12ml
1 - 10ml 3 - 8ml 1 - 3ml
5 - 1ml 3 - 6ml 1 - 2ml
1 - 2ml 3 - 4ml
1 - 3ml 1 - 71ml
1 - 4ml 1 - 65ml
1 - 5ml 1 - 24ml

Explanation / Answer

1 - 10ml 3 - 8ml 1 - 3ml

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