You\'ve recently discovered the sequences for two alleles for the mouse gene XCD
ID: 227564 • Letter: Y
Question
You've recently discovered the sequences for two alleles for the mouse gene XCD, which you have creatively named XCD^1 and XCD^2. This gene is believed to be involved in fat metabolism, somehow, but you haven't successfully been able to isolate the protein yet. You've also noticed that your mutant grey coat mice tend to be much leaner and muscular than those with the standard white coat. So for your pilot descriptive experiment with this gene, you'd like to determine if one coat color is more likely to have one allele over another. You're going to explore this hypothesis using a Southern blot. Why is a Southern blot the better choice than a Western blot at this point of knowledge about the gene? (1-2 sentences) You've already isolated and purified DNA samples from both grey and white mice and have your gel set ups ready to go. What else do you need to do prepare for your Southern blot? Your results are presented here for both XCD^1 and XCD^2 alleles. Based on this pilot information, do you think it's likely that different alleles for the XCD gene could contribute to lean less/body wide energy balance? Explain your answer. (2-3 sentences)Explanation / Answer
a. At this point of knowledge, Southern blot is used since ,the presence of a DNA sequence in a DNA sample must be checked. Western blot is used to sort proteins by siae or charge etc..Here we want to check for the gene for coat colour
b.The requirements for Souther blot include, a nitrocellulose membrane(to move the DNA from gel to the membrane), UV light and a Labelled DNA probe( for hybridization)
c. Based on the results seen on the blot, its not likely that two different alleles contribute to leanless/wide body. Both the alleles occupy the same position on the nylon paper , after blotting.
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