3. Make .8 H Mgso4.7H2 M 246.5 g Refer to this section when you need molecular w

Question

3. Make .8 H Mgso4.7H2 M 246.5 g Refer to this section when you need molecular weights to answer the problems. 4. Make 1 M KH PO 5. Make 1 M K2HPO 6. Make .250 M Na,HP0A MW-142 g 7, Make .01% Tris 8. Make 1% NaHCO 9. Make 10% CaCl2·2H20 10. Make .5M glucose 11. Make 50 ml 100 mM EDTA MW 292 g 12. Make 4 liters of Tank buffer for protein gel electrophoresis Mw = 136.1 g 2 4 Mw = 174.2 g = 121 g Mw = 84 g NW=111 g Mw = 180 g containing 0.025 M Tris, 0.192 M glycine (75.1 g/H) and 0.1% SDS (using a 10% stock solution of SDS).

Explanation / Answer

3.

Magnesium sulfate (MgSO4 • 7H2O) - for 0.1 M it's 24.7 g

so for 0.8M it'll be 197.6 g

12 .

Tris: mol.mass of tris = 121,1 g/mol: Tris = 0,025*4*121,1 g = 12,11 g for 4 L

Glycine: 0,192*75,1*4 = 57,67 g for 4L

SDS: Dilute stock solution 1/100 to get 0.1 % = 10 mL stock/L = 40 mL stock for 4 L

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