3. Lewis Science Center has been vandalized! The three suspects are Larry, Moe a

Question

3. Lewis Science Center has been vandalized! The three suspects are Larry, Moe and Curley. The culprit cuts themself, leaving behind blood and DNA. The IDIDIT locus in humans is an STR marker commonly used in DNA fingerprinting. The IDIDIT locus contains repeats of the nucleotides CAGAG. Forward and reverse primers corresponding to the 20 bp directly upstream and downstream from the repeats are used to amplify the region in a PCR reaction. The gel below shows the bands corresponding to the IDIDIT locus amplified from the blood left behind at the crime scene. Analysis of the suspects' DNA revealed that Moe has 12 and 20 repeats, Curley has 12 and 7 repeats and Moe has 7 and 2 repeats at the IDIDIT locus. Which one committed the crime? Blood Standard -= 175 - 150 125 - 100 75 - 50

Explanation / Answer

Answer - Moe or Larry is a suspect. In question they not mention about larry. The one who has 20 and 12 repeats is a suspect.

Because IDIDIT locus has a repeat CAGAG - 5 bp So in Moe or larry Blood has 20 repeats = 20 X 5 = 100 and 12 X 5 = 60 it is approximately equal to the blood sample. It is match with the Blood taken from the scene

Others curley has 12 X5= 60 and 7 X 5= 35

Larry or moe - 7 X 5 =35 and 2 X 5 = 10bp

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