Your PCR product was sequenced by a method known as Cycle Sequencing. Which of t

Question

Your PCR product was sequenced by a method known as Cycle Sequencing. Which of the following statements is false.

A. Tagged terminator nucleotides facilitate the creation of a series of nested DNA sequences of different length.

B. Cycle sequencing is done in a PCR machine.

C. Cycle sequencing can be completed in just one test tube.

DAn automatic sequencer performs electrophoresis and reads the tagged DNA pieces, providing a read out of the nucleotide bases comprising the DNA sequence of the fragment being tested.

Explanation / Answer

In cycle sequencing, four test tubes are required.

One test tube for each ddNTP.

Hence third statement is incorrect.

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