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a. cysteine protease b. metalloprotease c. threonine protease d. serine protease

ID: 225750 • Letter: A

Question

a. cysteine protease b. metalloprotease c. threonine protease d. serine protease e. aspartic protease Match the each following protein tags to their purification mechanism. "Strep" tag _______ FLAG ______ Inteins ______ a. Use of column containing chitin followed by self-cleavage. b. Use a specific antibody bound to a column or a bead. c. Use of streptavidin coated beads or column. d. Use a column containing nickel followed by imidazole. What is a recombinant protein? Match each or the recombinant protein products with its function. Factor VIII _______ Interleukin-2 ________ Erythropoietin _________ Somatotropin ____________ Tissue plasminogen factor _________ a. Promotes red blood cell formation. b. Treats growth hormone deficiency. c. Kills tumor cells. D. Dissolves blood clots to prevent heart attacks. e. Helps blood clots form in hemophiliacs. With respect to the following enzymes produced industrially, match each enzyme to its function. Invertase _______ Rennet _________ Catalase _________ Amylase ___________ Glucose isomerase _________ a. Degradation of starch, used in brewing. b. Protease specific to the cheese industry. c. Hydrolysis of sucrose to its monomers d. Conversion of glucose to fructose in high-fructose syrups. e Antioxidant used in processed foods. What is sited directed mutagenesis for?

Explanation / Answer

pepsin is a aspartic protease using aspargine in its catalytic site

strep tag- use of streptavidin coated beads

flag-use of antibody bound to a specific column or bead

intein-use od column containing chitin followed by cleavage

factor8 -forms blood clot

somatotropin- growth hormone

erytropoetin-RBC formation

Interleukin 2-kills tumor cell

TPA- Ddissolves blood clot

Recombinant proteins are produced fron rDNA in a cell.